Specimen and Sample Processing
Plants and Fungi
Organizing specimens and, more specifically, arraying them in a set pattern that mirrors a 96-well plate is a key step in DNA barcoding protocols. Properly arrayed specimens have a higher barcoding success and are less prone to error and contamination, complementing a medium to high throughput barcoding pipeline.
Complete and accurate specimen provenance information is important in multiple respects. Submission of this information to the Barcode of Life Data Systems (BOLD) is required to meet barcode standards and it is also a prerequisite for submitting samples to our Laboratory Information Management System and commencing molecular analysis. In addition, these data are very useful at the publication stage.
Images are a key component of DNA barcoding practices and there are standard protocols for submitting images to BOLD. In this course, we will review both SLR and microscopy imaging setups.
Barcoding protocols are optimized for a small amount of tissue/DNA. Consequently, most organisms which do not go through voucher recovery require tissue sampling according to standard procedures to avoid contamination.
For a detailed overview of the sampling process, click here.
It is important to keep detailed data records throughout the specimen processing procedure.
A collection voucher specimen preserved in an internationally recognized collection repository needs to accompany each barcode record. This should be available for re-examination by interested experts and for reference should there be any issues with the accompanying data.
Click here for a webinar presentation by International Development lead Dr. Alex Borisenko.